The glutathione peroxidase family of Theobroma cacao: Involvement in the oxidative stress during witches' broom disease.
Identifieur interne : 000070 ( Main/Exploration ); précédent : 000069; suivant : 000071The glutathione peroxidase family of Theobroma cacao: Involvement in the oxidative stress during witches' broom disease.
Auteurs : Akyla Maria Martins Alves [Brésil] ; Sara Pereira Menezes Reis [Brésil] ; Karina Peres Gramacho [Brésil] ; Fabienne Micheli [France]Source :
- International journal of biological macromolecules [ 1879-0003 ] ; 2020.
Abstract
The glutathione peroxidases (GPXs) are enzymes which are part of the cell antioxidant system inhibiting the ROS-induced damages of membranes and proteins. In cacao (Theobroma cacao L.) genome, five GPX genes were identified. Cysteine insertion codons (UGU) were found in TcPHGPX, TcGPX2, TcGPX4, TcGPX6 and tryptophan insertion codon (UGG) in TcGPX8. Multiple alignments revealed conserved domains between TcGPXs and other plants and human GPXs. Homology modeling was performed using the Populus trichocarpa GPX5 structure as template, and the molecular modeling showed that TcGPXs have affinity with selenometionine in their active site. In silico analysis of the TcGPXs promoter region revealed the presence of conserved cis-elements related to biotic stresses and hormone responsiveness. The expression analysis of TcGPXs in cacao plantlet meristems infected by M. perniciosa showed that TcGPXs are most expressed in susceptible variety than in resistant one, mainly in disease stages in which oxidative stress and programmed cell death occurred. This data, associated with phylogenetic and location analysis suggested that TcGPXs may play a role in protecting cells from oxidative stress as a try of disease progression reduction. To our knowledge, this is the first study of the overall GPX family from T. cacao.
DOI: 10.1016/j.ijbiomac.2020.08.222
PubMed: 32882281
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The glutathione peroxidase family of Theobroma cacao: Involvement in the oxidative stress during witches' broom disease.</title><author><name sortKey="Martins Alves, Akyla Maria" sort="Martins Alves, Akyla Maria" uniqKey="Martins Alves A" first="Akyla Maria" last="Martins Alves">Akyla Maria Martins Alves</name><affiliation wicri:level="2"><nlm:affiliation>Universidade Estadual de Santa Cruz (UESC), Departamento de Ciências Biológicas (DCB), Centro de Biotecnologia e Genética (CBG), Rodovia Ilhéus-Itabuna, km 16, 45662-900 Ilhéus, BA, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Universidade Estadual de Santa Cruz (UESC), Departamento de Ciências Biológicas (DCB), Centro de Biotecnologia e Genética (CBG), Rodovia Ilhéus-Itabuna, km 16, 45662-900 Ilhéus, BA</wicri:regionArea><placeName><region type="state">Bahia</region></placeName></affiliation></author><author><name sortKey="Pereira Menezes Reis, Sara" sort="Pereira Menezes Reis, Sara" uniqKey="Pereira Menezes Reis S" first="Sara" last="Pereira Menezes Reis">Sara Pereira Menezes Reis</name><affiliation wicri:level="2"><nlm:affiliation>Universidade Estadual de Santa Cruz (UESC), Departamento de Ciências Biológicas (DCB), Centro de Biotecnologia e Genética (CBG), Rodovia Ilhéus-Itabuna, km 16, 45662-900 Ilhéus, BA, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Universidade Estadual de Santa Cruz (UESC), Departamento de Ciências Biológicas (DCB), Centro de Biotecnologia e Genética (CBG), Rodovia Ilhéus-Itabuna, km 16, 45662-900 Ilhéus, BA</wicri:regionArea><placeName><region type="state">Bahia</region></placeName></affiliation></author><author><name sortKey="Peres Gramacho, Karina" sort="Peres Gramacho, Karina" uniqKey="Peres Gramacho K" first="Karina" last="Peres Gramacho">Karina Peres Gramacho</name><affiliation wicri:level="2"><nlm:affiliation>Cocoa Research Center, CEPLAC/CEPEC, 45600-970 Itabuna, BA, Brazil. Electronic address: karina@cepec.gov.br.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Cocoa Research Center, CEPLAC/CEPEC, 45600-970 Itabuna, BA</wicri:regionArea><placeName><region type="state">Bahia</region></placeName></affiliation></author><author><name sortKey="Micheli, Fabienne" sort="Micheli, Fabienne" uniqKey="Micheli F" first="Fabienne" last="Micheli">Fabienne Micheli</name><affiliation wicri:level="3"><nlm:affiliation>Universidade Estadual de Santa Cruz (UESC), Departamento de Ciências Biológicas (DCB), Centro de Biotecnologia e Genética (CBG), Rodovia Ilhéus-Itabuna, km 16, 45662-900 Ilhéus, BA, Brazil; CIRAD, UMR AGAP, F-34398 Montpellier, France. Electronic address: fabienne.micheli@cirad.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Universidade Estadual de Santa Cruz (UESC), Departamento de Ciências Biológicas (DCB), Centro de Biotecnologia e Genética (CBG), Rodovia Ilhéus-Itabuna, km 16, 45662-900 Ilhéus, BA, Brazil; CIRAD, UMR AGAP, F-34398 Montpellier</wicri:regionArea><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Languedoc-Roussillon</region><settlement type="city">Montpellier</settlement></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32882281</idno><idno type="pmid">32882281</idno><idno type="doi">10.1016/j.ijbiomac.2020.08.222</idno><idno type="wicri:Area/Main/Corpus">000119</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000119</idno><idno type="wicri:Area/Main/Curation">000119</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000119</idno><idno type="wicri:Area/Main/Exploration">000119</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The glutathione peroxidase family of Theobroma cacao: Involvement in the oxidative stress during witches' broom disease.</title><author><name sortKey="Martins Alves, Akyla Maria" sort="Martins Alves, Akyla Maria" uniqKey="Martins Alves A" first="Akyla Maria" last="Martins Alves">Akyla Maria Martins Alves</name><affiliation wicri:level="2"><nlm:affiliation>Universidade Estadual de Santa Cruz (UESC), Departamento de Ciências Biológicas (DCB), Centro de Biotecnologia e Genética (CBG), Rodovia Ilhéus-Itabuna, km 16, 45662-900 Ilhéus, BA, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Universidade Estadual de Santa Cruz (UESC), Departamento de Ciências Biológicas (DCB), Centro de Biotecnologia e Genética (CBG), Rodovia Ilhéus-Itabuna, km 16, 45662-900 Ilhéus, BA</wicri:regionArea><placeName><region type="state">Bahia</region></placeName></affiliation></author><author><name sortKey="Pereira Menezes Reis, Sara" sort="Pereira Menezes Reis, Sara" uniqKey="Pereira Menezes Reis S" first="Sara" last="Pereira Menezes Reis">Sara Pereira Menezes Reis</name><affiliation wicri:level="2"><nlm:affiliation>Universidade Estadual de Santa Cruz (UESC), Departamento de Ciências Biológicas (DCB), Centro de Biotecnologia e Genética (CBG), Rodovia Ilhéus-Itabuna, km 16, 45662-900 Ilhéus, BA, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Universidade Estadual de Santa Cruz (UESC), Departamento de Ciências Biológicas (DCB), Centro de Biotecnologia e Genética (CBG), Rodovia Ilhéus-Itabuna, km 16, 45662-900 Ilhéus, BA</wicri:regionArea><placeName><region type="state">Bahia</region></placeName></affiliation></author><author><name sortKey="Peres Gramacho, Karina" sort="Peres Gramacho, Karina" uniqKey="Peres Gramacho K" first="Karina" last="Peres Gramacho">Karina Peres Gramacho</name><affiliation wicri:level="2"><nlm:affiliation>Cocoa Research Center, CEPLAC/CEPEC, 45600-970 Itabuna, BA, Brazil. Electronic address: karina@cepec.gov.br.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Cocoa Research Center, CEPLAC/CEPEC, 45600-970 Itabuna, BA</wicri:regionArea><placeName><region type="state">Bahia</region></placeName></affiliation></author><author><name sortKey="Micheli, Fabienne" sort="Micheli, Fabienne" uniqKey="Micheli F" first="Fabienne" last="Micheli">Fabienne Micheli</name><affiliation wicri:level="3"><nlm:affiliation>Universidade Estadual de Santa Cruz (UESC), Departamento de Ciências Biológicas (DCB), Centro de Biotecnologia e Genética (CBG), Rodovia Ilhéus-Itabuna, km 16, 45662-900 Ilhéus, BA, Brazil; CIRAD, UMR AGAP, F-34398 Montpellier, France. Electronic address: fabienne.micheli@cirad.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Universidade Estadual de Santa Cruz (UESC), Departamento de Ciências Biológicas (DCB), Centro de Biotecnologia e Genética (CBG), Rodovia Ilhéus-Itabuna, km 16, 45662-900 Ilhéus, BA, Brazil; CIRAD, UMR AGAP, F-34398 Montpellier</wicri:regionArea><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Languedoc-Roussillon</region><settlement type="city">Montpellier</settlement></placeName></affiliation></author></analytic><series><title level="j">International journal of biological macromolecules</title><idno type="eISSN">1879-0003</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The glutathione peroxidases (GPXs) are enzymes which are part of the cell antioxidant system inhibiting the ROS-induced damages of membranes and proteins. In cacao (Theobroma cacao L.) genome, five GPX genes were identified. Cysteine insertion codons (UGU) were found in TcPHGPX, TcGPX2, TcGPX4, TcGPX6 and tryptophan insertion codon (UGG) in TcGPX8. Multiple alignments revealed conserved domains between TcGPXs and other plants and human GPXs. Homology modeling was performed using the Populus trichocarpa GPX5 structure as template, and the molecular modeling showed that TcGPXs have affinity with selenometionine in their active site. In silico analysis of the TcGPXs promoter region revealed the presence of conserved cis-elements related to biotic stresses and hormone responsiveness. The expression analysis of TcGPXs in cacao plantlet meristems infected by M. perniciosa showed that TcGPXs are most expressed in susceptible variety than in resistant one, mainly in disease stages in which oxidative stress and programmed cell death occurred. This data, associated with phylogenetic and location analysis suggested that TcGPXs may play a role in protecting cells from oxidative stress as a try of disease progression reduction. To our knowledge, this is the first study of the overall GPX family from T. cacao.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">32882281</PMID><DateRevised><Year>2020</Year><Month>11</Month><Day>10</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1879-0003</ISSN><JournalIssue CitedMedium="Internet"><Volume>164</Volume><PubDate><Year>2020</Year><Month>Dec</Month><Day>01</Day></PubDate></JournalIssue><Title>International journal of biological macromolecules</Title><ISOAbbreviation>Int J Biol Macromol</ISOAbbreviation></Journal><ArticleTitle>The glutathione peroxidase family of Theobroma cacao: Involvement in the oxidative stress during witches' broom disease.</ArticleTitle><Pagination><MedlinePgn>3698-3708</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0141-8130(20)34320-8</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.ijbiomac.2020.08.222</ELocationID><Abstract><AbstractText>The glutathione peroxidases (GPXs) are enzymes which are part of the cell antioxidant system inhibiting the ROS-induced damages of membranes and proteins. In cacao (Theobroma cacao L.) genome, five GPX genes were identified. Cysteine insertion codons (UGU) were found in TcPHGPX, TcGPX2, TcGPX4, TcGPX6 and tryptophan insertion codon (UGG) in TcGPX8. Multiple alignments revealed conserved domains between TcGPXs and other plants and human GPXs. Homology modeling was performed using the Populus trichocarpa GPX5 structure as template, and the molecular modeling showed that TcGPXs have affinity with selenometionine in their active site. In silico analysis of the TcGPXs promoter region revealed the presence of conserved cis-elements related to biotic stresses and hormone responsiveness. The expression analysis of TcGPXs in cacao plantlet meristems infected by M. perniciosa showed that TcGPXs are most expressed in susceptible variety than in resistant one, mainly in disease stages in which oxidative stress and programmed cell death occurred. This data, associated with phylogenetic and location analysis suggested that TcGPXs may play a role in protecting cells from oxidative stress as a try of disease progression reduction. To our knowledge, this is the first study of the overall GPX family from T. cacao.</AbstractText><CopyrightInformation>Copyright © 2020 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Martins Alves</LastName><ForeName>Akyla Maria</ForeName><Initials>AM</Initials><AffiliationInfo><Affiliation>Universidade Estadual de Santa Cruz (UESC), Departamento de Ciências Biológicas (DCB), Centro de Biotecnologia e Genética (CBG), Rodovia Ilhéus-Itabuna, km 16, 45662-900 Ilhéus, BA, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pereira Menezes Reis</LastName><ForeName>Sara</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Universidade Estadual de Santa Cruz (UESC), Departamento de Ciências Biológicas (DCB), Centro de Biotecnologia e Genética (CBG), Rodovia Ilhéus-Itabuna, km 16, 45662-900 Ilhéus, BA, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Peres Gramacho</LastName><ForeName>Karina</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Cocoa Research Center, CEPLAC/CEPEC, 45600-970 Itabuna, BA, Brazil. Electronic address: karina@cepec.gov.br.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Micheli</LastName><ForeName>Fabienne</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Universidade Estadual de Santa Cruz (UESC), Departamento de Ciências Biológicas (DCB), Centro de Biotecnologia e Genética (CBG), Rodovia Ilhéus-Itabuna, km 16, 45662-900 Ilhéus, BA, Brazil; CIRAD, UMR AGAP, F-34398 Montpellier, France. Electronic address: fabienne.micheli@cirad.fr.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>08</Month><Day>31</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Int J Biol Macromol</MedlineTA><NlmUniqueID>7909578</NlmUniqueID><ISSNLinking>0141-8130</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Gene expression</Keyword><Keyword MajorTopicYN="N">Moniliophthora perniciosa</Keyword><Keyword MajorTopicYN="N">Programmed cell death</Keyword></KeywordList><CoiStatement>Declaration of competing interest No conflicts of interest to declare.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>01</Month><Day>09</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>05</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>08</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>9</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>9</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>9</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32882281</ArticleId><ArticleId IdType="pii">S0141-8130(20)34320-8</ArticleId><ArticleId IdType="doi">10.1016/j.ijbiomac.2020.08.222</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Brésil</li><li>France</li></country><region><li>Bahia</li><li>Languedoc-Roussillon</li><li>Occitanie (région administrative)</li></region><settlement><li>Montpellier</li></settlement></list><tree><country name="Brésil"><region name="Bahia"><name sortKey="Martins Alves, Akyla Maria" sort="Martins Alves, Akyla Maria" uniqKey="Martins Alves A" first="Akyla Maria" last="Martins Alves">Akyla Maria Martins Alves</name></region><name sortKey="Pereira Menezes Reis, Sara" sort="Pereira Menezes Reis, Sara" uniqKey="Pereira Menezes Reis S" first="Sara" last="Pereira Menezes Reis">Sara Pereira Menezes Reis</name><name sortKey="Peres Gramacho, Karina" sort="Peres Gramacho, Karina" uniqKey="Peres Gramacho K" first="Karina" last="Peres Gramacho">Karina Peres Gramacho</name></country><country name="France"><region name="Occitanie (région administrative)"><name sortKey="Micheli, Fabienne" sort="Micheli, Fabienne" uniqKey="Micheli F" first="Fabienne" last="Micheli">Fabienne Micheli</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000070 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000070 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PoplarV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:32882281
|texte= The glutathione peroxidase family of Theobroma cacao: Involvement in the oxidative stress during witches' broom disease.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:32882281" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PoplarV1
| This area was generated with Dilib version V0.6.37. |  |